électrophorèse sur gel de polyacrylamide en présence de SDS

Traductions

• anglais

  Auteur : Conseil de recherches médicales du Canada, 1986

  Définition

  A technique in which charged complexes of protein with sodium dodecyl sulfate (SDS) are subjected to zone electrophoresis in a gel of uniform porosity /(polyacrylamide gel)/, the rate of migration increasing as the molecular weight decreases.

  Note :

  The relationship between the molecular weight of the protein and its mobility is linear, and can be used to estimate the protein's molecular weight. SDS denatures the protein and dissociates it into its subunits to form long, rodlike SDS-polypeptide complexes.

  Termes :

  1. SDS-gel electrophoresis
  2. SDS-polyacrylamide gel electrophoresis
  3. electrophoresis in polyacrylamide-SDS gels